|
KMID : 0894520180220030225
|
|
Development & Reproduction
2018 Volume.22 No. 3 p.225 ~ p.234
|
|
|
Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
|
|
Kim Ju-Won
Oh Bang-Geun
Kim Ju-Lan
Kim Dong-Gyun
Nam Bo-Hye
Kim Young-Ok
Park Jung-Youn
Cheong Jae-Hun
Kong Hee-Jeong
|
|
|
Abstract
|
|
|
|
A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
|
|
|
KEYWORD
|
|
|
Olive flounder, Embryo, Primary cell culture, Cell line
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|